Independent regulation of two genes in Escherichia coli by tetracyclines and Tet repressor variants.
نویسندگان
چکیده
We report a regulation system in Escherichia coli for independent regulation of two distinct reporter genes by application of Tet repressors with different specificities. One Tet repressor variant comprises wild-type tet operator (tetO) recognition and exclusive induction with the novel inducer 4-dedimethylamino-anhydrotetracycline. The other Tet repressor variant shows tetO-4C recognition and induction with tetracycline. We demonstrate that both variants are independently active in vivo and allow selective regulation of two genes in the same cell without any cross talk.
منابع مشابه
Evaluation of resistance genes in Escherichia coli isolated from toilets in the Islamic Azad University, Mashhad and genome sequencing (tet B, tet A, tet R, OXA 10 and OXA 48) in tetracycline-resistant strains
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 186 13 شماره
صفحات -
تاریخ انتشار 2004